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How Cyplasin™ kills cancer cells Melanoma cells can be genetically modified to express fluorescent tubulin (the basic unit of microtubules). With this technique, it is possible to see microtubules in the living cells directly and to observe how Cyplasin works. Below are images showing this process in action.
Summary of the process Cyplasin attaches itself to tumor-specific receptors on the outer cellular membrane. It induces a depolymerization of microtubules and a hyperpolymerization of actin filaments. The resulting over contraction leads irreversibly to the death of the cells. All these events occur at Cyplasin concentrations that are thought not to be harmful to normal cells. Other data generated demonstrate the selectivity of Cyplasin-SC™ for melanoma and other cancer cell types.
Cell death of melanoma cells after exposure to Cyplasin-SC. Cyplasin concentrations 60 nM and 30 nM kill at least an 80% of melanoma cells. These cells are highly sensitive to Cyplasin. Normal human primary cells maintain metabolic activity after exposure to Cyplasin-SC. When exposed to Cyplasin-SC for 24 hours, the normal human healthy cells (HUVEC cells) show 100% metabolic activity, demonstrating no cytotoxicity of Cyplasin-SC with these cells.
Other types of cancer cells have shown sensitivity to cyplasin as well while normal cells such as skin fibroblasts, neuronal cells insect and yeast cells do not. |
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